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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
5000.00 | 2.038 | 1.957 |
2500.00 | 1.407 | 1.326 |
1250.00 | 0.824 | 0.743 |
625.00 | 0.599 | 0.518 |
312.50 | 0.347 | 0.266 |
156.25 | 0.252 | 0.171 |
78.13 | 0.114 | 0.033 |
0.00 | 0.081 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 83-95% | 89% |
EDTA plasma(n=5) | 79-93% | 86% |
Heparin plasma(n=5) | 92-105% | 98% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 97-105% | 84-96% | 87-98% | 95-102% |
EDTA plasma(n=5) | 79-97% | 90-99% | 95-103% | 89-97% |
Heparin plasma(n=5) | 85-94% | 79-92% | 87-101% | 82-90% |
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