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        人胰高血糖素(GC)酶聯(lián)免疫吸附檢測試劑盒
        ELK1081
        規(guī)格: 價(jià)格:
        48T ¥1680.00
        96T ¥2400.00

        Overview

        Product name: Human GC(Glucagon) ELISA Kit
        Reactivity: Human
        Alternative Names: GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone
        Assay Type: Competitive Inhibition
        Sensitivity: 7.54 pg/mL
        Standard: 1500 pg/mL
        Detection Range: 23.44-1500 pg/mL
        Sample Type: serum, plasma and other biological fluids
        Assay Length: 2h
        Research Area: Endocrinology;
        Uniprot ID: P01275
        Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GC in the samples is then determined by comparing the OD of the samples to the standard curve.

        標(biāo)準(zhǔn)曲線

        Concentration (pg/mL) OD Corrected OD
        1500.00 0.191
        750.00 0.275
        375.00 0.446
        187.50 0.598
        93.75 0.763
        46.88 1.201
        23.44 1.624
        0.00 2.028

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant GC and the recovery rates were calculated by comparing the measured value to the expected amount of GC in samples.
        Matrix Recovery range Average
        serum(n=5) 93-105% 99%
        EDTA plasma(n=5) 84-93% 89%
        Heparin plasma(n=5) 81-103% 87%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 89-97% 85-97% 87-96% 85-96%
        EDTA plasma(n=5) 95-103% 89-101% 87-98% 85-92%
        Heparin plasma(n=5) 85-97% 86-102% 87-99% 99-105%
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