CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VE-Cadherin, and the Human VE-Cadherin standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VE-Cadherin. After TMB substrate solution is added, only those wells that contain Human VE-Cadherin and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.
標(biāo)準(zhǔn)曲線(xiàn)
Concentration (μg/mL)
OD
Corrected OD
10.00
2.075
1.987
5.00
1.608
1.520
2.50
1.205
1.117
1.25
0.852
0.764
0.63
0.514
0.426
0.32
0.317
0.229
0.16
0.252
0.164
0.00
0.088
0.000
精密度
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant VE-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of VE-Cadherin in samples.
Matrix
Recovery range
Average
serum(n=5)
85-97%
91%
EDTA plasma(n=5)
80-95%
87%
Heparin plasma(n=5)
89-103%
96%
線(xiàn)性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VE-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.