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一步法人白介素2受體a鏈(IL-2Ra/CD25)酶聯(lián)免疫吸附檢測試劑盒
ELK087ES 規(guī)格： |
 價(jià)格： |
|---|---|
| 48T | ￥2240.00 |
| 96T | ￥3200.00 |
下載說明 ①Overview
標(biāo)準(zhǔn)曲線
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 6000.00 | 2.189 | 2.088 |
| 3000.00 | 1.582 | 1.481 |
| 1500.00 | 1.243 | 1.142 |
| 750.00 | 0.935 | 0.834 |
| 375.00 | 0.503 | 0.402 |
| 187.50 | 0.285 | 0.184 |
| 93.75 | 0.219 | 0.118 |
| 0.00 | 0.101 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant IL-2Ra/CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2Ra/CD25 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 81-97% | 89% |
| EDTA plasma(n=5) | 85-99% | 92% |
| Heparin plasma(n=5) | 90-105% | 97% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2Ra/CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 79-96% | 85-99% | 79-93% | 89-97% |
| EDTA plasma(n=5) | 88-105% | 86-92% | 81-93% | 79-97% |
| Heparin plasma(n=5) | 85-102% | 87-96% | 92-101% | 89-104% |
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