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Concentration (pg/mL) | OD | Corrected OD |
---|---|---|
400.00 | 1.951 | 1.856 |
200.00 | 1.522 | 1.427 |
100.00 | 1.088 | 0.993 |
50.00 | 0.805 | 0.710 |
25.00 | 0.506 | 0.411 |
12.50 | 0.317 | 0.222 |
6.25 | 0.176 | 0.081 |
0.00 | 0.095 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 95-105% | 100% |
EDTA plasma(n=5) | 80-95% | 87% |
Heparin plasma(n=5) | 92-105% | 98% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-96% | 85-97% | 85-94% | 87-96% |
EDTA plasma(n=5) | 88-97% | 91-98% | 86-103% | 79-91% |
Heparin plasma(n=5) | 85-104% | 87-106% | 83-92% | 82-90% |
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