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Concentration (μg/mL) | OD | Corrected OD |
---|---|---|
100.00 | 0.219 | |
50.00 | 0.411 | |
25.00 | 0.692 | |
12.50 | 0.933 | |
6.25 | 1.119 | |
3.13 | 1.565 | |
1.57 | 1.813 | |
0.00 | 2.219 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-97% | 91% |
EDTA plasma(n=5) | 82-94% | 88% |
Heparin plasma(n=5) | 89-107% | 98% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 85-94% | 97-103% | 79-91% | 82-91% |
EDTA plasma(n=5) | 95-102% | 83-96% | 97-103% | 85-92% |
Heparin plasma(n=5) | 91-104% | 82-96% | 88-101% | 90-99% |
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