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一步法人基質(zhì)金屬蛋白酶8(MMP8)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK070ES

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	EasyStep Human MMP8(Matrix Metalloproteinase 8) ELISA Kit
Reactivity:	Human
Alternative Names:	CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase
Assay Type:	Sandwich
Sensitivity:	24 pg/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Enzyme & Kinase;Infection immunity;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP-8, and the Human MMP-8 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MMP-8. After TMB substrate solution is added, only those wells that contain Human MMP-8 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP-8 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線(xiàn)

Concentration (ng/mL)	OD	Corrected OD
10.00	2.231	2.127
5.00	1.697	1.593
2.50	1.069	0.965
1.25	0.752	0.648
0.63	0.451	0.347
0.32	0.352	0.248
0.16	0.228	0.124
0.00	0.104	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MMP-8 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP-8 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	90-103%	96%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP-8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.