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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
20.00 | 2.154 | 2.061 |
10.00 | 1.556 | 1.463 |
5.00 | 1.108 | 1.015 |
2.50 | 0.895 | 0.802 |
1.25 | 0.509 | 0.416 |
0.63 | 0.302 | 0.209 |
0.32 | 0.225 | 0.132 |
0.00 | 0.093 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 82-95% | 88% |
EDTA plasma(n=5) | 81-95% | 88% |
Heparin plasma(n=5) | 90-103% | 96% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 96-105% | 88-96% | 87-98% | 93-101% |
EDTA plasma(n=5) | 89-97% | 85-92% | 95-103% | 87-93% |
Heparin plasma(n=5) | 95-106% | 88-103% | 81-98% | 93-102% |
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