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        小鼠三碘甲狀腺原氨酸(T3)酶聯(lián)免疫吸附檢測(cè)試劑盒
        
                    ELK0554
                    
        
                        
        
                            
        
                                規(guī)格：
                                價(jià)格：
                            
        
                                                        
        
                                48T
                                ￥1680.00
                            
        
                                                        
        
                                96T
                                ￥2400.00
                            
        
                                                    
        
                    
        
                    
                
        
            
        
        
        

                
        
            
        
                
        Overview
                    文獻(xiàn)
        
                
        
                    
        
                        
        
                            
        
                                
        
                                    
        
                                        Product name:
                                        Mouse T3(Triiodothyronine) ELISA Kit
                                    
        
                                                                            
        
                                            Reactivity:
                                            Mouse
                                        
        
                                                                                
        
                                            Alternative Names:
                                            3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin
                                        
        
                                                                                
        
                                           Assay Type:
                                           Competitive Inhibition
                                           
                                        
        
                                                                                
        
                                           Sensitivity:
                                           6.14 pg/mL
                                           
                                        
        
                                                                                
        
                                           Standard:
                                           1000 pg/mL
                                           
                                        
        
                                                                                
        
                                           Detection Range:
                                           15.63-1000 pg/mL
                                           
                                        
        
                                                                                
        
                                           Sample Type:
                                           serum, plasma and other biological fluids
                                           
                                        
        
                                                                                
        
                                           Assay Length:
                                           2.5h
                                           
                                        
        
                                                                                
        
                                           Research Area:
                                           Endocrinology;Autoimmunity;
                                           
                                        
        
                                                                                
        
                                           Test principle:
                                           This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse T3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse T3 in the samples is then determined by comparing the OD of the samples to the standard curve.
                                           
                                        
        
                                                                        
        
                            
        
                                                        
                                                    
        
                    
        
                
        
            
        
                        
        
                
        標(biāo)準(zhǔn)曲線
        
                
        
                    
        
                        
        
                            
        
                                Concentration (pg/mL)
                                OD
                                                                Corrected OD
                                                            
        
                                                        
        
                                1000.00
                                0.191
                                                                
                                                            
        
                                                        
        
                                500.00
                                0.357
                                                                
                                                            
        
                                                        
        
                                250.00
                                0.577
                                                                
                                                            
        
                                                        
        
                                125.00
                                0.842
                                                                
                                                            
        
                                                        
        
                                62.50
                                1.275
                                                                
                                                            
        
                                                        
        
                                31.25
                                1.578
                                                                
                                                            
        
                                                        
        
                                15.63
                                1.769
                                                                
                                                            
        
                                                        
        
                                0.00
                                2.265
                                                                
                                                            
        
                                                    
        
                    
        

                    
        
                
        
            
        
                        
        
                
        精密度
        
                
        
                    
        Intra-assay Precision (Precision within an assay)：CV%<8%
        
                    
        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
        
                    
        Inter-assay Precision (Precision between assays)：CV%<10%
        
                    
        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
        
                
        
            
        
                        
        
                
        回收率
        
                
        
                    
        Matrices listed below were spiked with certain level of recombinant T3 and the recovery rates were calculated by comparing the measured value to the expected amount of T3 in samples.
        
                    
        
                        
        
                            
        
                                Matrix
                                Recovery range
                                Average
                            
        
                            
        
                                serum(n=5)
                                80-95%
                                87%
                            
        
                            
        
                                EDTA plasma(n=5)
                                85-97%
                                91%
                            
        
                            
        
                                Heparin plasma(n=5)
                                92-107%
                                99%
                            
        
                        
        
                    
        
                
        
            
        
                        
        
                
        線性
        
                
        
                    
        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of T3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        
                    
        
                        
        
                            
        
                                Matrix
                                1:2
                                1:4
                                1:8
                                1:16
                            
        
                            
        
                                serum(n=5)
                                88-97%
                                96-103%
                                86-92%
                                89-97%
                            
        
                            
        
                                EDTA plasma(n=5)
                                85-94%
                                87-101%
                                86-97%
                                88-95%
                            
        
                            
        
                                Heparin plasma(n=5)
                                85-94%
                                97-106%
                                92-101%
                                82-90%
                            
        
                        
        
                    
        
                
        
            
        
                    
        
    
        
    




        
    
        
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