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        一步法大鼠25-羥基維生素D(25-OH-D)酶聯(lián)免疫吸附檢測試劑盒
        ELK053ES
        規(guī)格: 價格:
        48T ¥2660.00
        96T ¥3800.00

        Overview

        Product name: EasyStep Rat 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
        Reactivity: Rat
        Alternative Names: 25 Hydroxy Vitamin D;
        Assay Type: Competitive Inhibition
        Sensitivity: 7.7 ng/mL
        Standard: 5000 ng/mL
        Detection Range: 78.13-5000 ng/mL
        Sample Type: Serum, plasma
        Assay Length: 1.5h
        Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
        Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 25-OH-D antigen, and the Rat 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

        標準曲線

        Concentration (ng/mL) OD Corrected OD
        5000.00 0.189
        2500.00 0.397
        1250.00 0.583
        625.00 0.833
        312.50 1.157
        156.25 1.583
        78.13 1.715
        0.00 2.211

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant 25-OH-D and the recovery rates were calculated by comparing the measured value to the expected amount of 25-OH-D in samples.
        Matrix Recovery range Average
        serum(n=5) 85-97% 91%
        EDTA plasma(n=5) 91-105% 98%
        Heparin plasma(n=5) 95-107% 101%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-OH-D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 85-107% 84-98% 95-103% 86-98%
        EDTA plasma(n=5) 86-93% 91-105% 87-98% 99-102%
        Heparin plasma(n=5) 85-97% 87-96% 86-98% 91-103%

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