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下載說明 ①Overview
標準曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 5000.00 | 0.221 | |
| 2500.00 | 0.411 | |
| 1250.00 | 0.711 | |
| 625.00 | 0.959 | |
| 312.50 | 1.253 | |
| 156.25 | 1.579 | |
| 78.13 | 1.761 | |
| 0.00 | 2.375 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant 25-OH-D and the recovery rates were calculated by comparing the measured value to the expected amount of 25-OH-D in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 78-93% | 85% |
| EDTA plasma(n=5) | 87-99% | 93% |
| Heparin plasma(n=5) | 87-95% | 91% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-OH-D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-98% | 92-104% | 82-96% | 95-102% |
| EDTA plasma(n=5) | 90-99% | 83-97% | 81-103% | 89-101% |
| Heparin plasma(n=5) | 88-98% | 87-103% | 89-97% | 85-94% |
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