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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
100.00 | 0.189 | |
50.00 | 0.386 | |
25.00 | 0.611 | |
12.50 | 0.823 | |
6.25 | 1.169 | |
3.13 | 1.592 | |
1.57 | 1.697 | |
0.00 | 2.213 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 90-103% | 96% |
EDTA plasma(n=5) | 87-99% | 93% |
Heparin plasma(n=5) | 85-97% | 91% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-102% | 87-96% | 92-101% | 83-96% |
EDTA plasma(n=5) | 85-97% | 93-101% | 97-103% | 91-99% |
Heparin plasma(n=5) | 86-93% | 93-101% | 85-98% | 90-101% |
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