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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
80.00 | 1.948 | 1.855 |
40.00 | 1.642 | 1.549 |
20.00 | 1.139 | 1.046 |
10.00 | 0.797 | 0.704 |
5.00 | 0.527 | 0.434 |
2.50 | 0.319 | 0.226 |
1.25 | 0.227 | 0.134 |
0.00 | 0.093 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-99% | 92% |
EDTA plasma(n=5) | 78-92% | 85% |
Heparin plasma(n=5) | 78-90% | 84% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 86-95% | 82-93% | 97-106% | 89-102% |
EDTA plasma(n=5) | 85-94% | 87-103% | 82-94% | 78-90% |
Heparin plasma(n=5) | 95-106% | 89-102% | 87-98% | 85-92% |
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