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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
80.00 | 2.096 | 2.014 |
40.00 | 1.562 | 1.480 |
20.00 | 1.288 | 1.206 |
10.00 | 0.975 | 0.893 |
5.00 | 0.567 | 0.485 |
2.50 | 0.326 | 0.244 |
1.25 | 0.215 | 0.133 |
0.00 | 0.082 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 90-105% | 97% |
EDTA plasma(n=5) | 80-97% | 88% |
Heparin plasma(n=5) | 78-92% | 85% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 95-102% | 85-96% | 90-98% | 82-93% |
EDTA plasma(n=5) | 85-94% | 87-101% | 82-95% | 82-93% |
Heparin plasma(n=5) | 95-103% | 79-96% | 93-106% | 87-98% |
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