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一步法人含NLR家族Pyrin域蛋白3(NLRP3)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK039ES

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說(shuō)明 ①

Overview

Product name:	EasyStep Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit
Reactivity:	Human
Alternative Names:	AVP; AGTAVPRL; AII; AII/AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCU; MWS; NALP3; PYPAF1; Cryopyrin; Cold Induced Autoinflammatory Syndrome 1; Caterpiller-Like Receptor 1.1
Assay Type:	Sandwich
Sensitivity:	37 pg/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Signal transduction;
Uniprot ID:	D4A523
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NLRP3, and the Human NLRP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NLRP3. After TMB substrate solution is added, only those wells that contain Human NLRP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NLRP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線(xiàn)

Concentration (ng/mL)	OD	Corrected OD
10.00	2.156	2.071
5.00	1.679	1.594
2.50	1.196	1.111
1.25	0.835	0.750
0.63	0.582	0.497
0.32	0.327	0.242
0.16	0.199	0.114
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NLRP3 and the recovery rates were calculated by comparing the measured value to the expected amount of NLRP3 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	80-97%	88%
Heparin plasma(n=5)	83-95%	89%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NLRP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.