• <th id="abimu"></th>

      1. 產(chǎn)品中心

        一步法人氨基端前腦鈉素(NT-ProBNP)酶聯(lián)免疫吸附檢測試劑盒
        ELK035ES
        規(guī)格: 價(jià)格:
        48T ¥1960.00
        96T ¥2800.00

        Overview

        Product name: EasyStep Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
        Reactivity: Human
        Alternative Names: NT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
        Assay Type: Sandwich
        Sensitivity: 0.94 ng/mL
        Standard: 60 ng/mL
        Detection Range: 0.94-60 ng/mL
        Sample Type: Serum, plasma
        Assay Length: 1.5h
        Research Area: Endocrinology;Cardiovascular biology;
        Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP, and the Human NT-ProBNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NT-ProBNP. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

        標(biāo)準(zhǔn)曲線

        Concentration (ng/mL) OD Corrected OD
        60.00 2.207 2.106
        30.00 1.643 1.542
        15.00 1.251 1.150
        7.50 0.847 0.746
        3.75 0.625 0.524
        1.88 0.301 0.200
        0.94 0.288 0.187
        0.00 0.101 0.000

        精密度

        Intra-assay Precision (Precision within an assay):CV%<8%

        Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

        Inter-assay Precision (Precision between assays):CV%<10%

        Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

        回收率

        Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
        Matrix Recovery range Average
        serum(n=5) 90-103% 96%
        EDTA plasma(n=5) 81-96% 88%
        Heparin plasma(n=5) 78-94% 86%

        線性

        The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
        Matrix 1:2 1:4 1:8 1:16
        serum(n=5) 89-98% 83-103% 98-105% 92-101%
        EDTA plasma(n=5) 96-101% 85-92% 79-96% 89-101%
        Heparin plasma(n=5) 93-105% 85-97% 90-99% 82-101%

        產(chǎn)品成分表

        關(guān)閉

        在線咨詢

        Online consultation

        • 在線咨詢
        • 技術(shù)支持

        關(guān)注微信公眾號

        微信掃一掃立即咨詢

        微信掃一掃立即咨詢

        關(guān)閉
        一级a爱片国产亚洲精品,97se亚洲综合色区,日朝欧美亚洲精品,欧洲韩国亚洲一区二区三区

      2. <th id="abimu"></th>