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標(biāo)準(zhǔn)曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 60.00 | 2.207 | 2.106 |
| 30.00 | 1.643 | 1.542 |
| 15.00 | 1.251 | 1.150 |
| 7.50 | 0.847 | 0.746 |
| 3.75 | 0.625 | 0.524 |
| 1.88 | 0.301 | 0.200 |
| 0.94 | 0.288 | 0.187 |
| 0.00 | 0.101 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-103% | 96% |
| EDTA plasma(n=5) | 81-96% | 88% |
| Heparin plasma(n=5) | 78-94% | 86% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 89-98% | 83-103% | 98-105% | 92-101% |
| EDTA plasma(n=5) | 96-101% | 85-92% | 79-96% | 89-101% |
| Heparin plasma(n=5) | 93-105% | 85-97% | 90-99% | 82-101% |
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